Product Info Summary
SKU: | A03587-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-BRMS1 Antibody Picoband™
SKU/Catalog Number
A03587-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BRMS1 Antibody Picoband™ catalog # A03587-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BRMS1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03587-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human BRMS1 recombinant protein (Position: D52-D244). Human BRMS1 shares 93.6% and 96% amino acid (aa) sequence identity with mouse and rat BRMS1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03587-1 is reactive to BRMS1 in Human, Mouse, Rat
Applications
A03587-1 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
34 kDa
Calculated molecular weight
28.461kDa
Background of BRMS1
Breast cancer metastasis-suppressor 1 is a protein that in humans is encoded by the BRMS1 gene. This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of BRMS1 using anti-BRMS1 antibody (A03587-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates,
Lane 4: HELA whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRMS1 antigen affinity purified polyclonal antibody (Catalog # A03587-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRMS1 at approximately 34KD. The expected band size for BRMS1 is at 28KD.
Protein Target Info & Infographic
Gene/Protein Information For BRMS1 (Source: Uniprot.org, NCBI)
Gene Name
BRMS1
Full Name
Breast cancer metastasis-suppressor 1
Weight
28.461kDa
Superfamily
BRMS1 family
Alternative Names
breast cancer metastasis suppressor 1; breast cancer metastasis-suppressor 1; DKFZP564A063 BRMS1 BRMS1 transcriptional repressor and anoikis regulator breast cancer metastasis-suppressor 1|breast cancer metastasis suppressor 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BRMS1, check out the BRMS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BRMS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BRMS1 Antibody Picoband™ (A03587-1)
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No publications found for A03587-1
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Customer Reviews
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